6j0h | pdb_00006j0h

Crystal structure of Actinomycin D- d(TTGGCGAA) complex

spinqualitylabelsall models 6j0h, resolution 1.52Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6j0h is a 2 chain structure with sequence from Streptomyces sp. and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.52Å Ligands: DVA, MVA, NA, PXZ, SAR Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed DNA mismatches are highly polymorphic and dynamic in nature, albeit poorly characterized structurally. We utilized the antitumour antibiotic CoII(Chro)2 (Chro = chromomycin A3) to stabilize the palindromic duplex d(TTGGCGAA) DNA with two G:G mismatches, allowing X-ray crystallography-based monitoring of mismatch polymorphism. For the first time, the unusual geometry of several G:G mismatches including syn-syn, water mediated anti-syn and syn-syn-like conformations can be simultaneously observed in the crystal structure. The G:G mismatch sites of the d(TTGGCGAA) duplex can also act as a hotspot for the formation of alternative DNA structures with a GC/GA-5' intercalation site for binding by the GC-selective intercalator actinomycin D (ActiD). Direct intercalation of two ActiD molecules to G:G mismatch sites causes DNA rearrangements, resulting in backbone distortion to form right-handed Z-DNA structures with a single-step sharp kink. Our study provides insights on intercalators-mismatch DNA interactions and a rationale for mismatch interrogation and detection via DNA intercalation. Polymorphic G:G mismatches act as hotspots for inducing right-handed Z DNA by DNA intercalation.,Satange R, Chuang CY, Neidle S, Hou MH Nucleic Acids Res. 2019 Jul 30. pii: 5541096. doi: 10.1093/nar/gkz653. PMID:31361900 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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