7o7z

Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (classified for pseudoknot)

spinqualitylabelsall models 7o7z, resolution 2.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7o7z is a 10 chain structure with sequence from Bos taurus and Oryctolagus cuniculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 2.4Å Ligands: 1MA, 2MG, 4AC, 5MC, 5MU, 6MZ, A2M, AAC, AME, AYA, B8N, G7M, GTP, H2U, HIC, HY3, M2G, M3L, MA6, MG, MLZ, N, NMM, OMC, OMG, OMU, PSU, SAC, SPD, SPM, UNX, UR3, UY1, V5N, YYG, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function G1TZ76_RABIT Publication Abstract from PubMed Programmed ribosomal frameshifting is a key event during translation of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA genome that allows synthesis of the viral RNA-dependent RNA polymerase and downstream proteins. Here, we present the cryo-electron microscopy structure of a translating mammalian ribosome primed for frameshifting on the viral RNA. The viral RNA adopts a pseudoknot structure that lodges at the entry to the ribosomal messenger RNA (mRNA) channel to generate tension in the mRNA and promote frameshifting, whereas the nascent viral polyprotein forms distinct interactions with the ribosomal tunnel. Biochemical experiments validate the structural observations and reveal mechanistic and regulatory features that influence frameshifting efficiency. Finally, we compare compounds previously shown to reduce frameshifting with respect to their ability to inhibit SARS-CoV-2 replication, establishing coronavirus frameshifting as a target for antiviral intervention. Structural basis of ribosomal frameshifting during translation of the SARS-CoV-2 RNA genome.,Bhatt PR, Scaiola A, Loughran G, Leibundgut M, Kratzel A, Meurs R, Dreos R, O'Connor KM, McMillan A, Bode JW, Thiel V, Gatfield D, Atkins JF, Ban N Science. 2021 Jun 18;372(6548):1306-1313. doi: 10.1126/science.abf3546. Epub 2021 , May 13. PMID:34029205 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ribosome 3D structures 3D sructureseceptor for activated protein kinase C 1

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