5wwk | pdb_00005wwk

Highly stable green fluorescent protein

spinqualitylabelsall models 5wwk, resolution 3.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5wwk is a 6 chain structure with sequence from Aequorea victoria. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.199Å Ligands: CR2 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A059PIQ0_AEQVI Publication Abstract from PubMed Red fluorescent proteins with a large Stokes shift offer a limited autofluorescence background and are used in deep tissue imaging. Here, by introducing the free amino group in Aequorea victoria, the electrostatic charges of the p-hydroxybenzylidene imidazolinone chromophore of green fluorescent protein (GFP) have been altered resulting in an unusual, 85 nm red-shifted fluorescence. The structural and biophysical analysis suggested that the red shift is due to positional shift occupancy of Glu222 and Arg96, resulting in extended conjugation and a relaxed chromophore. Femtosecond transient absorption spectra exhibited that the excited state relaxation dynamics of red-shifted GFP (rGFP) (tau4 = 234 ps) are faster compared to the A. victoria green fluorescent protein (tau4 = 3.0 ns). The nanosecond time-resolved emission spectra of rGFP reveal the continuous spectral shift during emission by a solvent reorientation in the chromophore. Finally, the molecular dynamics simulations revealed the rearrangement of the hydrogen bond interactions in the chromophore vicinity, reshaping the symmetric distribution of van der Waals space to fine tune the GFP structure resulting from highly red-shifted rGFP. Excited State Electronic Interconversion and Structural Transformation of Engineered Red-Emitting Green Fluorescent Protein Mutant.,Augustine G, Raghavan S, NumbiRamudu K, Easwaramoorthi S, Shanmugam G, Seetharani Murugaiyan J, Gunasekaran K, Govind C, Karunakaran V, Ayyadurai N J Phys Chem B. 2019 Mar 14;123(10):2316-2324. doi: 10.1021/acs.jpcb.8b10516. Epub, 2019 Mar 6. PMID:30789731 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Green Fluorescent Protein 3D structures

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