5x7h | pdb_00005x7h

Crystal Structure of Paenibacillus sp. 598K cycloisomaltooligosaccharide glucanotransferase complexed with cycloisomaltoheptaose

spinqualitylabelsall models 5x7h, resolution 2.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5x7h is a 1 chain structure with sequence from Paenibacillus sp. 598K. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.6Å Ligands: CA, GLC, MLI, NA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function G9MBW2_9BACL Publication Abstract from PubMed Paenibacillus sp. 598K cycloisomaltooligosaccharide glucanotransferase (PsCITase), a member of glycoside hydrolase family 66, catalyzes the intramolecular transglucosylation of dextran to produce cycloisomaltooligosaccharides (CIs) with seven or more degrees of polymerization. To clarify the cyclization reaction and product specificity of the enzyme, we determined the crystal structure of PsCITase. The core structure of PsCITase consists of four structural domains: a catalytic (beta/alpha)8-domain and three beta-domains. A family 35 carbohydrate-binding module (PsCBM35-1) is inserted into and protrudes from the catalytic domain. The ligand complex structure of PsCITase prepared by soaking the crystal with cycloisomaltoheptaose yielded bound sugars at three sites: in the catalytic cleft; at the joint of the PsCBM35-1 domain; and at the loop region of PsCBM35-1. In the catalytic site, soaked cycloisomaltoheptaose was observed as a linear isomaltoheptaose, presumably a hydrolyzed product from cycloisomaltoheptaose by the enzyme, and occupied subsites -7 to -1. Beyond subsite -7, three glucose moieties of another isomaltooiligosaccharide were observed, and these positions are considered to be distal subsites -11 to -13. The third binding site is the canonical sugar-binding site at the loop region of PsCBM35-1, where the soaked cycloisomaltoheptaose was bound. The structure indicated that the concave surface between the catalytic domain and PsCBM35-1 plays a guiding route for the long-chained substrate at the cyclization reaction. Isomaltooligosaccharide-binding structure of Paenibacillus sp. 598K cycloisomaltooligosaccharide glucanotransferase.,Fujimoto Z, Kishine N, Suzuki N, Suzuki R, Mizushima D, Momma M, Kimura K, Funane K Biosci Rep. 2017 Apr 6. pii: BSR20170253. doi: 10.1042/BSR20170253. PMID:28385816 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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