6ihd | pdb_00006ihd

Crystal structure of Malate dehydrogenase from Metallosphaera sedula

spinqualitylabelsall models 6ihd, resolution 2.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6ihd is a 2 chain structure with sequence from Metallosphaera sedula. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.3Å Ligands: ETX, NAD Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A088E2H7_9CREN Publication Abstract from PubMed Metallosphaera sedula is a thermoacidophilic autotrophic archaeon and known to utilize the 3-hydroxypropionate/4-hydroxybutyrate cycle (3-HP/4-HB cycle) as a carbon fixation pathway. The 3-HP/4-HB cycle in M. sedula is associated with central metabolism, and malate dehydrogenase (MDH) is an enzyme involved in the central metabolism that converts malate to oxaloacetate. To elucidate the enzymatic properties of MDH from M. sedula (MsMDH), we determined the crystal structure of MsMDH as a complex with NAD(+) and a ternary complex with malate and NAD(+). Based on its complex structures and biochemical experiments, we observed that MsMDH can utilize both NAD(+) and NADP(+) as a cofactor. In addition, we revealed that MsMDH shows a conformational change at the active site upon substrate binding. Based on the comparison with other MDHs, we revealed that MsMDH was distinguished from general MDHs due to a Lys80 residue, and this difference is likely to influence the unique cofactor specificity of MsMDH. Crystal structure and biochemical characterization of malate dehydrogenase from Metallosphaera sedula.,Lee D, Hong J, Kim KJ Biochem Biophys Res Commun. 2019 Feb 12;509(3):833-838. doi:, 10.1016/j.bbrc.2019.01.018. Epub 2019 Jan 10. PMID:30638660 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Malate Dehydrogenase 3D structures

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