6itp | pdb_00006itp

Crystal structure of cortisol complexed with its nanobody at pH 3.5

spinqualitylabelsall models 6itp, resolution 1.57Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6itp is a 2 chain structure with sequence from Camelus bactrianus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.572Å Ligands: HCY Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed To date, few structural models of VHH antibody binding to low molecular weight haptens have been reported. Here, we report the crystal structure of cortisol binding to its VHH antibody NbCor at pH 3.5 and 10.5. Cortisol binds to NbCor mainly by burying itself under the tunnel formed by the complementarity determining region 1 (CDR1) of NbCor. The affinity of NbCor binding to cortisol and similar compounds was also verified by a microscale thermophoresis assay. Combining our findings with several previously reported structures of hapten-VHH antibody complexes, we propose that VHH antibodies exhibit a special mechanism of binding small haptens by encapsulating them in a tunnel formed by CDR1. Our findings provide useful structural information for the further development and optimization of hapten-specific VHH antibodies. Structural insights into the mechanism of single domain VHH antibody binding to cortisol.,Ding L, Wang Z, Zhong P, Jiang H, Zhao Z, Zhang Y, Ren Z, Ding Y FEBS Lett. 2019 Jun;593(11):1248-1256. doi: 10.1002/1873-3468.13398. Epub 2019, May 20. PMID:31049949 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Antibody 3D structures 3D structures of non-human antibody

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