6jym | pdb_00006jym

Crystal structure of Prolyl Endopeptidase from Haliotis discus hannai

spinqualitylabelsall models 6jym, resolution 1.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6jym is a 1 chain structure with sequence from Haliotis discus hannai. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.5Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A1X9T5X9_HALDH Publication Abstract from PubMed Aimed to study the characteristics of prolyl endopeptidase (PEP, EC 3.4.21.26) and its possible role in the degradation of collagen, we cloned the full-length cDNA sequence of PEP from abalone (Haliotis discus hannai) (Hdh-PEP). Recombinant Hdh-PEP (rHdh-PEP) was expressed in vitro, its enzymatic properties were detected, and its secondary structure was analyzed by Circular Dichroism (CD). We for the first time determined the 1.5 A crystal structure of rHdh-PEP. The decomposition effect of rHdh-PEP on collagen peptides was analyzed. Our data revealed that the molecular weight of rHdh-PEP is 85 kDa, consisting of a catalytic domain and a beta-propeller domain. The optimal pH and temperature of rHdh-PEP were pH 6.0 and 20 degrees C, respectively. Using small collagen peptides as substrates, HPLC-ESI-MS analysis confirmed that rHdh-PEP specifically cleaved at the carboxyl side of proline residues, suggesting its role in the degradation of collagen peptides during autolysis. Characterization and crystal structure of prolyl endopeptidase from abalone (Haliotis discus hannai).,Li WY, Li Y, Chen YL, Hu JJ, Mengist HM, Liu GM, Jin T, Cao MJ Food Chem. 2020 Dec 15;333:127452. doi: 10.1016/j.foodchem.2020.127452. Epub 2020, Jul 4. PMID:32673951 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Prolyl Endopeptidase

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