2mc5 | pdb_00002mc5

A bacteriophage transcription regulator inhibits bacterial transcription initiation by -factor displacement

spinqualitylabelsall models 2mc5 Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2mc5 is a 1 chain structure with sequence from Xanthomonas virus Xp10. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Solution NMR Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q8LTJ5_9CAUD Publication Abstract from PubMed Bacteriophages (phages) appropriate essential processes of bacterial hosts to benefit their own development. The multisubunit bacterial RNA polymerase (RNAp) enzyme, which catalyses DNA transcription, is targeted by phage-encoded transcription regulators that selectively modulate its activity. Here, we describe the structural and mechanistic basis for the inhibition of bacterial RNAp by the transcription regulator P7 encoded by Xanthomonas oryzae phage Xp10. We reveal that P7 uses a two-step mechanism to simultaneously interact with the catalytic beta and beta' subunits of the bacterial RNAp and inhibits transcription initiation by inducing the displacement of the sigma70-factor on initial engagement of RNAp with promoter DNA. The new mode of interaction with and inhibition mechanism of bacterial RNAp by P7 underscore the remarkable variety of mechanisms evolved by phages to interfere with host transcription. A bacteriophage transcription regulator inhibits bacterial transcription initiation by sigma-factor displacement.,Liu B, Shadrin A, Sheppard C, Mekler V, Xu Y, Severinov K, Matthews S, Wigneshweraraj S Nucleic Acids Res. 2014 Jan 30. PMID:24482445 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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