6m4c | pdb_00006m4c
C. albicans actin interacting protein Aip5
Structural highlights
FunctionPublication Abstract from PubMedCandida albicans is a dimorphic fungus that converts from a yeast form to a hyphae form during infection. This switch requires the formation of an actin cable to coordinate polarized cell growth. It's known that nucleation of this cable requires a multiprotein complex localized at the tip called the polarisome, but the mechanisms underpinning this process were unclear. Here, we found that C. albicans Aip5, a homolog of polarisome component ScAip5 in S. cerevisiae that nucleates actin polymerization and synergizes with the formin ScBni1, regulates actin assembly and hyphae growth synergistically with other polarisome proteins Bni1, Bud6, and Spa2. The C-terminus of Aip5 binds directly to G-actin, Bni1 and the C-terminal of Bud6, which form the core of the nucleation complex to polymerize F-actin. Based on insights from structural biology and molecular dynamic simulations, we propose a possible complex conformation of the actin nucleation core, which provides cooperative positioning and supports the synergistic actin nucleation activity of a tri-protein complex Bni1-Bud6-Aip5. Together with known interactions of Bni1 with Bud6 and Aip5 in S. cerevisiae, our findings unravel molecular mechanisms of C. albicans by which the tri-protein complex coordinates the actin nucleation in actin cable assembly and hyphal growth, which is likely a conserved mechanism in different filamentous fungi and yeast. Orchestrated actin nucleation by the Candida albicans polarisome complex enables filamentous growth.,Xie Y, Loh ZY, Xue J, Zhou F, Sun J, Qiao Z, Jin S, Deng Y, Li H, Wang Y, Lu L, Gao YG, Miao Y J Biol Chem. 2020 Aug 26. pii: RA120.013890. doi: 10.1074/jbc.RA120.013890. PMID:32848016[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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