6m63 | pdb_00006m63

Crystal structure of a cAMP sensor G-Flamp1.

spinqualitylabelsall models 6m63, resolution 2.25Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6m63 is a 2 chain structure with sequence from Aequorea victoria and Mesorhizobium japonicum MAFF 303099. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.25Å Ligands: CMP, CRO Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function CNGK1_RHILO Cyclic nucleotide-regulated potassium channel activated by cAMP. GFP_AEQVI Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. Publication Abstract from PubMed cAMP is a key second messenger that regulates diverse cellular functions including neural plasticity. However, the spatiotemporal dynamics of intracellular cAMP in intact organisms are largely unknown due to low sensitivity and/or brightness of current genetically encoded fluorescent cAMP indicators. Here, we report the development of the new circularly permuted GFP (cpGFP)-based cAMP indicator G-Flamp1, which exhibits a large fluorescence increase (a maximum DeltaF/F0 of 1100% in HEK293T cells), decent brightness, appropriate affinity (a Kd of 2.17 muM) and fast response kinetics (an association and dissociation half-time of 0.20 and 0.087 s, respectively). Furthermore, the crystal structure of the cAMP-bound G-Flamp1 reveals one linker connecting the cAMP-binding domain to cpGFP adopts a distorted beta-strand conformation that may serve as a fluorescence modulation switch. We demonstrate that G-Flamp1 enables sensitive monitoring of endogenous cAMP signals in brain regions that are implicated in learning and motor control in living organisms such as fruit flies and mice. A high-performance genetically encoded fluorescent indicator for in vivo cAMP imaging.,Wang L, Wu C, Peng W, Zhou Z, Zeng J, Li X, Yang Y, Yu S, Zou Y, Huang M, Liu C, Chen Y, Li Y, Ti P, Liu W, Gao Y, Zheng W, Zhong H, Gao S, Lu Z, Ren PG, Ng HL, He J, Chen S, Xu M, Li Y, Chu J Nat Commun. 2022 Sep 12;13(1):5363. doi: 10.1038/s41467-022-32994-7. PMID:36097007 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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