7d2y | pdb_00007d2y

complex of two RRM domains

Structural highlights

7d2y is a 4 chain structure with sequence from Caenorhabditis elegans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.68Å
Ligands:	SO4
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TOFU6_CAEEL Component of the pid-1 and tost-1 variants of the PETISCO complexes, which have roles in the biogenesis of a class of 21 nucleotide PIWI-interacting RNAs (piRNAs) that possess a uracil residue at the 5'-end (also called 21U-RNAs) and embryogenesis, respectively (PubMed:31147388, PubMed:31216475). Promotes the biogenesis of 21U-RNAs (PubMed:31216475). Required for chromosome segregation and cell division in early embryos (PubMed:17171368, PubMed:31216475). May have a role in DNA replication (PubMed:17171368).^[1] ^[2] ^[3] ^[4]

Publication Abstract from PubMed

By incorporating two mutually exclusive factors, PID-1 and TOST-1, C. elegans PICS complex plays important roles in piRNA biogenesis, chromosome segregation and cell division. We firstly map the interaction network between PICS subunits, then uncover the mechanisms underlying the interactions between PICS subunits by solving several complex structures, including those of TOFU-6/PICS-1, ERH-2/PICS-1, and ERH-2/TOST-1. Our biochemical experiment also demonstrates that PICS exists as an octamer consisting of two copies of each subunit. Combining structural analyses with mutagenesis experiments, we identify interfacial residues of PICS subunits that are critical for maintaining intact PICS complex in vitro. Furthermore, using genetics, cell biology and imaging experiments, we find that those mutants impairing the in vitro interaction network within PICS, also lead to dysfunction of PICS in vivo, including mislocalization of PICS, and reduced levels of piRNAs or aberrant chromosome segregation and cell division. Therefore, our work provides structural insights into understanding the PICS-mediated piRNA biogenesis and cell division.

Molecular basis for PICS-mediated piRNA biogenesis and cell division.,Wang X, Zeng C, Liao S, Zhu Z, Zhang J, Tu X, Yao X, Feng X, Guang S, Xu C Nat Commun. 2021 Sep 22;12(1):5595. doi: 10.1038/s41467-021-25896-7. PMID:34552083^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Minasaki R, Streit A. MEL-47, a novel protein required for early cell divisions in the nematode Caenorhabditis elegans. Mol Genet Genomics. 2007 Mar;277(3):315-28. PMID:17171368 doi:10.1007/s00438-006-0191-z
↑ Cordeiro Rodrigues RJ, de Jesus Domingues AM, Hellmann S, Dietz S, de Albuquerque BFM, Renz C, Ulrich HD, Sarkies P, Butter F, Ketting RF. PETISCO is a novel protein complex required for 21U RNA biogenesis and embryonic viability. Genes Dev. 2019 Jul 1;33(13-14):857-870. doi: 10.1101/gad.322446.118. Epub 2019, May 30. PMID:31147388 doi:https://dx.doi.org/10.1101/gad.322446.118
↑ Zeng C, Weng C, Wang X, Yan YH, Li WJ, Xu D, Hong M, Liao S, Dong MQ, Feng X, Xu C, Guang S. Functional Proteomics Identifies a PICS Complex Required for piRNA Maturation and Chromosome Segregation. Cell Rep. 2019 Jun 18;27(12):3561-3572.e3. doi: 10.1016/j.celrep.2019.05.076. PMID:31216475 doi:https://dx.doi.org/10.1016/j.celrep.2019.05.076
↑ Zeng C, Weng C, Wang X, Yan YH, Li WJ, Xu D, Hong M, Liao S, Dong MQ, Feng X, Xu C, Guang S. Functional Proteomics Identifies a PICS Complex Required for piRNA Maturation and Chromosome Segregation. Cell Rep. 2019 Jun 18;27(12):3561-3572.e3. doi: 10.1016/j.celrep.2019.05.076. PMID:31216475 doi:https://dx.doi.org/10.1016/j.celrep.2019.05.076
↑ Wang X, Zeng C, Liao S, Zhu Z, Zhang J, Tu X, Yao X, Feng X, Guang S, Xu C. Molecular basis for PICS-mediated piRNA biogenesis and cell division. Nat Commun. 2021 Sep 22;12(1):5595. doi: 10.1038/s41467-021-25896-7. PMID:34552083 doi:https://dx.doi.org/10.1038/s41467-021-25896-7

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Minasaki R, Streit A. MEL-47, a novel protein required for early cell divisions in the nematode Caenorhabditis elegans. Mol Genet Genomics. 2007 Mar;277(3):315-28. PMID:17171368 doi:10.1007/s00438-006-0191-z

[2] Cordeiro Rodrigues RJ, de Jesus Domingues AM, Hellmann S, Dietz S, de Albuquerque BFM, Renz C, Ulrich HD, Sarkies P, Butter F, Ketting RF. PETISCO is a novel protein complex required for 21U RNA biogenesis and embryonic viability. Genes Dev. 2019 Jul 1;33(13-14):857-870. doi: 10.1101/gad.322446.118. Epub 2019, May 30. PMID:31147388 doi:https://dx.doi.org/10.1101/gad.322446.118

[3] Zeng C, Weng C, Wang X, Yan YH, Li WJ, Xu D, Hong M, Liao S, Dong MQ, Feng X, Xu C, Guang S. Functional Proteomics Identifies a PICS Complex Required for piRNA Maturation and Chromosome Segregation. Cell Rep. 2019 Jun 18;27(12):3561-3572.e3. doi: 10.1016/j.celrep.2019.05.076. PMID:31216475 doi:https://dx.doi.org/10.1016/j.celrep.2019.05.076

[4] Zeng C, Weng C, Wang X, Yan YH, Li WJ, Xu D, Hong M, Liao S, Dong MQ, Feng X, Xu C, Guang S. Functional Proteomics Identifies a PICS Complex Required for piRNA Maturation and Chromosome Segregation. Cell Rep. 2019 Jun 18;27(12):3561-3572.e3. doi: 10.1016/j.celrep.2019.05.076. PMID:31216475 doi:https://dx.doi.org/10.1016/j.celrep.2019.05.076

[5] Wang X, Zeng C, Liao S, Zhu Z, Zhang J, Tu X, Yao X, Feng X, Guang S, Xu C. Molecular basis for PICS-mediated piRNA biogenesis and cell division. Nat Commun. 2021 Sep 22;12(1):5595. doi: 10.1038/s41467-021-25896-7. PMID:34552083 doi:https://dx.doi.org/10.1038/s41467-021-25896-7

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7d2y | pdb_00007d2y

complex of two RRM domains

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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