7ffa | pdb_00007ffa

Diarylpentanoid-producing polyketide synthase from Aquilaria sinensis

spinqualitylabelsall models 7ffa, resolution 1.98Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7ffa is a 2 chain structure with sequence from Aquilaria sinensis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.98Å Ligands: CSD Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A385MEG6_9ROSI Publication Abstract from PubMed 2-(2-Phenylethyl)chromones (PECs) are the principal constituents contributing to the distinctive fragrance of agarwood. How PECs are biosynthesized is currently unknown. In this work, we describe a diarylpentanoid-producing polyketide synthase (PECPS) identified from Aquilaria sinensis. Through biotransformation experiments using fluorine-labeled substrate, transient expression of PECPS in Nicotiana benthamiana, and knockdown of PECPS expression in A. sinensis calli, we demonstrate that the C6-C5-C6 scaffold of diarylpentanoid is the common precursor of PECs, and PECPS plays a crucial role in PECs biosynthesis. Crystal structure (1.98 A) analyses and site-directed mutagenesis reveal that, due to its small active site cavity (247 A(3)), PECPS employs a one-pot formation mechanism including a "diketide-CoA intermediate-released" step for the formation of the C6-C5-C6 scaffold. The identification of PECPS, the pivotal enzyme of PECs biosynthesis, provides insight into not only the feasibility of overproduction of pharmaceutically important PECs using metabolic engineering approaches, but also further exploration of how agarwood is formed. Identification of a diarylpentanoid-producing polyketide synthase revealing an unusual biosynthetic pathway of 2-(2-phenylethyl)chromones in agarwood.,Wang XH, Gao BW, Nakashima Y, Mori T, Zhang ZX, Kodama T, Lee YE, Zhang ZK, Wong CP, Liu QQ, Qi BW, Wang J, Li J, Liu X, Abe I, Morita H, Tu PF, Shi SP Nat Commun. 2022 Jan 17;13(1):348. doi: 10.1038/s41467-022-27971-z. PMID:35039506 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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