7vm8 | pdb_00007vm8

Crystal structure of the MtDMI1 gating ring

spinqualitylabelsall models 7vm8, resolution 3.03Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7vm8 is a 1 chain structure with sequence from Medicago truncatula. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.034Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function DMI1_MEDTR Required for early signal transduction events leading to endosymbiosis. Acts early in a signal transduction chain leading from the perception of Nod factor to the activation of calcium spiking. Also involved in mycorrhizal symbiosis. May be involved in the regulation of the calcium channel responsible for calcium spiking by mobilizing another cation, and thereby altering the membrane potential. Publication Abstract from PubMed Nuclear Ca(2+) oscillations allow symbiosis signaling, facilitating plant recognition of beneficial microsymbionts, nitrogen-fixing rhizobia, and nutrient-capturing arbuscular mycorrhizal fungi. Two classes of channels, DMI1 and CNGC15, in a complex on the nuclear membrane, coordinate symbiotic Ca(2+) oscillations. However, the mechanism of Ca(2+) signature generation is unknown. Here, we demonstrate spontaneous activation of this channel complex, through gain-of-function mutations in DMI1, leading to spontaneous nuclear Ca(2+) oscillations and spontaneous nodulation, in a CNGC15-dependent manner. The mutations destabilize a hydrogen-bond or salt-bridge network between two RCK domains, with the resultant structural changes, alongside DMI1 cation permeability, activating the channel complex. This channel complex was reconstituted in human HEK293T cell lines, with the resultant calcium influx enhanced by autoactivated DMI1 and CNGC15s. Our results demonstrate the mode of activation of this nuclear channel complex, show that DMI1 and CNGC15 are sufficient to create oscillatory Ca(2+) signals, and provide insights into its native mode of induction. Constitutive activation of a nuclear-localized calcium channel complex in Medicago truncatula.,Liu H, Lin JS, Luo Z, Sun J, Huang X, Yang Y, Xu J, Wang YF, Zhang P, Oldroyd GED, Xie F Proc Natl Acad Sci U S A. 2022 Aug 23;119(34):e2205920119. doi:, 10.1073/pnas.2205920119. Epub 2022 Aug 16. PMID:35972963 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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