7voh | pdb_00007voh
The a-glucosidase QsGH13 from Qipengyuania seohaensis
Structural highlights
Publication Abstract from PubMedThe alpha-glucosidases play indispensable roles in the metabolic mechanism of organism, prevention, and treatment of the disease, and sugar hydrolysis, and are widely used in chemical synthesis, clinical diagnosis, and other fields. However, improving their catalytic efficiency and production to meet commercial demand remains a huge challenge. Here we detected a novel GH13 family alpha-glucosidase, QsGH13, from the deep-sea bacterium Qipengyuania seohaensis sp. SW-135. QsGH13 is highly substrate specific and only hydrolyzes sugars containing alpha-1,4 glucoside bonds. For example, its enzymatic activity for p-nitrophenyl-alpha-D-glucopyranoside was 25.41 U/mg, and the K (m) value was 0.2952 +/- 0.0322 mM. The biochemical results showed that the optimum temperature of QsGH13 is 45 degrees C, the optimum pH is 10.0, and it has excellent biological characteristics such as alkali resistance and salt resistance. The crystal structure of QsGH13 was resolved with a resolution of 2.2 A, where QsGH13 is composed of a typical TIM barrel catalytic domain A, a loop-rich domain B, and a conserved domain C. QsGH13 crystal belonged to the monoclinic space group P2(1)2(1)2(1), with unit-cell parameters a = 58.816 A, b = 129.920 A, c = 161.307 A, alpha = gamma = beta = 90 degrees , which contains two monomers per asymmetric unit. The beta --> alpha loop 4 of QsGH13 was located above catalytic pocket. Typical catalytic triad residues Glu202, Asp266, and Glu329 were found in QsGH13. The biochemical properties and structural analysis of QsGH13 have greatly improved our understanding of the catalytic mechanism of GH13 family. This study provides new ideas to broaden the application of alpha-glucosidase in alcohol fermentation, glycolysis, and other industries. Structure and Function Insight of the alpha-Glucosidase QsGH13 From Qipengyuania seohaensis sp. SW-135.,Zhai X, Wu K, Ji R, Zhao Y, Lu J, Yu Z, Xu X, Huang J Front Microbiol. 2022 Mar 3;13:849585. doi: 10.3389/fmicb.2022.849585. , eCollection 2022. PMID:35308395[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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