7wcf | pdb_00007wcf

Crystal structure of the kinase with AMP-PNP

spinqualitylabelsall models 7wcf, resolution 1.36Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7wcf is a 1 chain structure with sequence from Legionella pneumophila subsp. pneumophila str. Philadelphia 1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.3636Å Ligands: ACP, MG, SEP Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q5ZSZ6_LEGPH Publication Abstract from PubMed Toxin-antitoxin (TA) systems are ubiquitous genetic modules in bacteria and archaea. Here, we perform structural and biochemical characterization of the Legionella pneumophila effector Lpg2370, demonstrating that it is a Ser/Thr kinase. Together with two upstream genes, lpg2370 constitutes the tripartite HipBST TA. Notably, the toxin Lpg2370 (HipTLp) and the antitoxin Lpg2369 (HipSLp) correspond to the C-terminus and N-terminus of HipA from HipBA TA, respectively. By determining crystal structures of autophosphorylated HipTLp, its complex with AMP-PNP, and the structure of HipTLp-HipSLp complex, we identify residues in HipTLp critical for ATP binding and those contributing to its interactions with HipSLp. Structural analysis reveals that HipSLp binding induces a loop-to-helix shift in the P-loop of HipTLp, leading to the blockage of ATP binding and inhibition of the kinase activity. These findings establish the L. pneumophila effector Lpg2370 as the HipBST TA toxin and elucidate the molecular basis for HipT neutralization in HipBST TA. Molecular mechanism of toxin neutralization in the HipBST toxin-antitoxin system of Legionella pneumophila.,Zhen X, Wu Y, Ge J, Fu J, Ye L, Lin N, Huang Z, Liu Z, Luo ZQ, Qiu J, Ouyang S Nat Commun. 2022 Jul 26;13(1):4333. doi: 10.1038/s41467-022-32049-x. PMID:35882877 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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