8jh1 | pdb_00008jh1

Crystal Structure of the Csm6 Y161A mutant from Thermus thermophilus HB8 in complex with cyclic-tetraadenylate (cA4)

spinqualitylabelsall models 8jh1, resolution 2.89Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8jh1 is a 3 chain structure with sequence from Thermus thermophilus HB8 and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.89Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function CSM6_THET8 CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). The type III-A Csm effector complex binds crRNA and acts as a crRNA-guided RNase, DNase and cyclic oligoadenylate synthase; binding of target RNA cognate to the crRNA is required for all activities. This protein is not part of the Csm effector complex (Probable). A single-strand-specific endoribonuclease (ssRNase) producing free 5'-OH (PubMed:26763118). Activity is approximately 1000-fold stimulated by cyclic oligoadenylate (cOA); only cyclic tetraadenylate (cA4) stimulates the ssRNase activity while linear oligoadenylates do not activate the RNase (PubMed:28663439). Another study showed stimulation by linear tetraadenylate at very high concentrations, but did not examine stimulation by cA4 (PubMed:28722012).

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