2j4r | pdb_00002j4r

Structural Study of the Aquifex aeolicus PPX-GPPA enzyme

spinqualitylabelsall models 2j4r, resolution 2.71Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2j4r is a 2 chain structure with sequence from Aquifex aeolicus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.71Å Ligands: G4P Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function O67040_AQUAE Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed The crystal structure of the prototype exopolyphosphatase/guanosine pentaphosphate phosphohydrolase protein family member from Aquifex aeolicus in complex with the intracellular second messenger guanosine tetraphosphate was determined at 2.7-A resolution. The hydrolytic base is identified as E119. The dual specificity established for the Escherichia coli homolog is shown to be compatible with a common active site for guanosine pentaphosphate and polyphosphate hydrolysis. Distinct and different degrees of closure between the two domains of the enzyme are associated with substrate binding. The arginines R22 and R267, residing in different domains, are crucial for guanosine pentaphosphate specificity as they interact with the unique 3'-ribose phosphorylation. Structure of the PPX/GPPA phosphatase from Aquifex aeolicus in complex with the alarmone ppGpp.,Kristensen O, Ross B, Gajhede M J Mol Biol. 2008 Feb 1;375(5):1469-76. Epub 2007 Dec 4. PMID:18155044 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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