2jk8 | pdb_00002jk8
Type IV secretion system effector protein BepA complexed with a pyrophosphate moiety
Structural highlights
FunctionBEPA_BARHE Adenylyltransferase involved in virulence by mediating the addition of adenosine 5'-monophosphate (AMP) to specific residue of host target proteins.[1] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedNumerous bacterial pathogens subvert cellular functions of eukaryotic host cells by the injection of effector proteins via dedicated secretion systems. The type IV secretion system (T4SS) effector protein BepA from Bartonella henselae is composed of an N-terminal Fic domain and a C-terminal BID domain, the latter being responsible for T4SS-mediated translocation into host cells. A proteolysis resistant fragment (residues 10 to 302) that includes the Fic domain shows auto-adenylylation activity and adenylyl transfer onto Hela cell extract proteins as demonstrated by autoradiography upon incubation with alpha-[(32)P]-ATP. Its crystal structure, determined to 2.9 A resolution by the SeMet-SAD method, exhibits the canonical Fic fold including the HPFxxGNGRxxR signature motif with several elaborations in loop regions and an additional beta-rich domain at the C-terminus. Upon crystal soaking with ATP/Mg(2+), additional electron density indicated the presence of a PP(i)/Mg(2+) moiety, the side product of the adenylylation reaction, in the anion binding nest of the signature motif. Based on this information and that of the recent structure of IbpA(Fic2) in complex with the eukaryotic target protein Cdc42, we present a detailed model for the ternary complex of Fic with the two substrates, ATP/Mg(2+) and target tyrosine. The model is consistent with an in-line nucleophilic attack of the deprotonated side-chain hydroxyl group onto the alpha-phosphorus of the nucleotide to accomplish AMP transfer. Furthermore, a general, sequence independent mechanism of target positioning through antiparallel beta-strand interactions between enzyme and target is suggested. Fic domain catalyzed adenylylation: Insight provided by the structural analysis of the type IV secretion system effector BepA.,Palanivelu DV, Goepfert A, Meury M, Guye P, Dehio C, Schirmer T Protein Sci. 2011 Jan 6. PMID:21213248[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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