8cib | pdb_00008cib

Structural and functional analysis of the Pseudomonas aeruginosa PA1677 protein

spinqualitylabelsall models 8cib, resolution 1.78Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8cib is a 6 chain structure with sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.78Å Ligands: ACT, LYS, MPD Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A072ZIR4_PSEAI Publication Abstract from PubMed In the opportunistic human pathogen Pseudomonas aeruginosa (Pae), carbon catabolite repression (CCR) orchestrates the hierarchical utilization of N and C sources, and impacts virulence, antibiotic resistance and biofilm development. During CCR, the RNA chaperone Hfq and the catabolite repression control protein Crc form assemblies on target mRNAs that impede translation of proteins involved in uptake and catabolism of less preferred C sources. After exhaustion of the preferred C-source, translational repression of target genes is relieved by the regulatory RNA CrcZ, which binds to and acts as a decoy for Hfq. Here, we asked whether Crc action can be modulated to relieve CCR after exhaustion of a preferred carbon source. As Crc does not bind to RNA per se, we endeavored to identify an interacting protein. In vivo co-purification studies, co-immunoprecipitation and biophysical assays revealed that Crc binds to Pae strain O1 protein PA1677. Our structural studies support bioinformatics analyzes showing that PA1677 belongs to the isochorismatase-like superfamily. Ectopic expression of PA1677 resulted in de-repression of Hfq/Crc controlled target genes, while in the absence of the protein, an extended lag phase is observed during diauxic growth on a preferred and a non-preferred carbon source. This observations indicate that PA1677 acts as an antagonist of Crc that favors synthesis of proteins required to metabolize non-preferred carbon sources. We present a working model wherein PA1677 diminishes the formation of productive Hfq/Crc repressive complexes on target mRNAs by titrating Crc. Accordingly, we propose the name CrcA (catabolite repression control protein antagonist) for PA1677. Catabolite repression control protein antagonist, a novel player in Pseudomonas aeruginosa carbon catabolite repression control.,Sonnleitner E, Bassani F, Cianciulli Sesso A, Brear P, Lilic B, Davidovski L, Resch A, Luisi BF, Moll I, Blasi U Front Microbiol. 2023 May 12;14:1195558. doi: 10.3389/fmicb.2023.1195558. , eCollection 2023. PMID:37250041 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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