3zzr | pdb_00003zzr
Crystal structure of the CG11501 protein in P21212 spacegroup
Structural highlights
FunctionDIE_DROME Cytokine which promotes survival following infection by Sindbis virus by suppressing the immune deficiency pathway (PubMed:26739560). Following infection by the enteropathogenic bacteria E.carotovora limits intestinal stem cells proliferation (PubMed:30036358). When secreted from muscle or adipose tissue, can attenuate age-related intestinal tissue degeneration by inhibiting apoptosis (PubMed:30036358).[1] [2] Publication Abstract from PubMedBACKGROUND: The Drosophila melanogaster gene CG11501 is up regulated after a septic injury and was proposed to act as a negative regulator of the JAK/STAT signaling pathway. Diedel, the CG11501 gene product, is a small protein of 115 residues with 10 cysteines. METHODOLOGY/PRINCIPAL FINDINGS: We have produced Diedel in Drosophila S2 cells as an extra cellular protein thanks to its own signal peptide and solved its crystal structure at 1.15 A resolution by SIRAS using an iodo derivative. Diedel is composed of two sub domains SD1 and SD2. SD1 is made of an antiparallel beta-sheet covered by an alpha-helix and displays a ferredoxin-like fold. SD2 reveals a new protein fold made of loops connected by four disulfide bridges. Further structural analysis identified conserved hydrophobic residues on the surface of Diedel that may constitute a potential binding site. The existence of two conformations, cis and trans, for the proline 52 may be of interest as prolyl peptidyl isomerisation has been shown to play a role in several physiological mechanisms. The genome of D. melanogaster contains two other genes coding for proteins homologous to Diedel, namely CG43228 and CG34329. Strikingly, apart from Drosophila and the pea aphid Acyrthosiphon pisum, Diedel-related sequences were exclusively identified in a few insect DNA viruses of the Baculoviridae and Ascoviridae families. CONCLUSION/SIGNIFICANCE: Diedel, a marker of the Drosophila antimicrobial/antiviral response, is a member of a small family of proteins present in drosophilids, aphids and DNA viruses infecting lepidopterans. Diedel is an extracellular protein composed of two sub-domains. Two special structural features (hydrophobic surface patch and cis/trans conformation for proline 52) may indicate a putative interaction site, and support an extra cellular signaling function for Diedel, which is in accordance with its proposed role as negative regulator of the JAK/STAT signaling pathway. Crystal structure of Diedel, a marker of the immune response of Drosophila melanogaster.,Coste F, Kemp C, Bobezeau V, Hetru C, Kellenberger C, Imler JL, Roussel A PLoS One. 2012;7(3):e33416. Epub 2012 Mar 19. PMID:22442689[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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