5owp | pdb_00005owp

Crystal structure of glycopeptide "GVTSAfPDT*RPAP" in complex with scFv-SM3

spinqualitylabelsall models 5owp, resolution 1.85Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5owp is a 2 chain structure with sequence from Mus musculus and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.85Å Ligands: 4FB, A2G, EDO Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function LV1D_MOUSE HVM32_MOUSE Publication Abstract from PubMed A structure-based design of a new generation of tumor-associated glycopeptides with improved affinity against two anti-MUC1 antibodies is described. These unique antigens feature a fluorinated proline residue, such as a (4S)-4-fluoro-l-proline or 4,4-difluoro-l-proline, at the most immunogenic domain. Binding assays using biolayer interferometry reveal 3-fold to 10-fold affinity improvement with respect to the natural (glyco)peptides. According to X-ray crystallography and MD simulations, the fluorinated residues stabilize the antigen-antibody complex by enhancing key CH/pi interactions. Interestingly, a notable improvement in detection of cancer-associated anti-MUC1 antibodies from serum of patients with prostate cancer is achieved with the non-natural antigens, which proves that these derivatives can be considered better diagnostic tools than the natural antigen for prostate cancer. The Use of Fluoroproline in MUC1 Antigen Enables Efficient Detection of Antibodies in Patients with Prostate Cancer.,Somovilla VJ, Bermejo IA, Albuquerque IS, Martinez-Saez N, Castro-Lopez J, Garcia-Martin F, Companon I, Hinou H, Nishimura SI, Jimenez-Barbero J, Asensio JL, Avenoza A, Busto JH, Hurtado-Guerrero R, Peregrina JM, Bernardes GJL, Corzana F J Am Chem Soc. 2017 Dec 12. doi: 10.1021/jacs.7b09447. PMID:29166012 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Antibody 3D structures Monoclonal Antibodies 3D structures 3D structures of non-human antibody

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