5nlm | pdb_00005nlm

Complex between a UDP-glucosyltransferase from Polygonum tinctorium capable of glucosylating indoxyl and indoxyl sulfate

spinqualitylabelsall models 5nlm, resolution 2.14Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5nlm is a 2 chain structure with sequence from Persicaria tinctoria. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.14Å Ligands: IOS, MG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A2R2JFJ4_9CARY Publication Abstract from PubMed Indigo is an ancient dye uniquely capable of producing the signature tones in blue denim; however, the dyeing process requires chemical steps that are environmentally damaging. We describe a sustainable dyeing strategy that not only circumvents the use of toxic reagents for indigo chemical synthesis but also removes the need for a reducing agent for dye solubilization. This strategy utilizes a glucose moiety as a biochemical protecting group to stabilize the reactive indigo precursor indoxyl to form indican, preventing spontaneous oxidation to crystalline indigo during microbial fermentation. Application of a beta-glucosidase removes the protecting group from indican, resulting in indigo crystal formation in the cotton fibers. We identified the gene coding for the glucosyltransferase PtUGT1 from the indigo plant Polygonum tinctorium and solved the structure of PtUGT1. Heterologous expression of PtUGT1 in Escherichia coli supported high indican conversion, and biosynthesized indican was used to dye cotton swatches and a garment. Employing a biochemical protecting group for a sustainable indigo dyeing strategy.,Hsu TM, Welner DH, Russ ZN, Cervantes B, Prathuri RL, Adams PD, Dueber JE Nat Chem Biol. 2018 Jan 8. pii: nchembio.2552. doi: 10.1038/nchembio.2552. PMID:29309053 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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