5ao8 | pdb_00005ao8

Crystal Structure of SltB3 from Pseudomonas aeruginosa in complex with NAG-NAM-pentapeptide

spinqualitylabelsall models 5ao8, resolution 2.23Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5ao8 is a 1 chain structure with sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.23Å Ligands: CA, NAG, NM9 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q9HX28_PSEAE Publication Abstract from PubMed A family of 11 lytic transglycosylases in Pseudomonas aeruginosa, an opportunistic human pathogen, turn over the polymeric bacterial cell wall in the course of its recycling, repair, and maturation. The functions of these enzymes are not fully understood. We disclose herein that SltB3 of P. aeruginosa is an exolytic lytic transglycosylase. We characterize its reaction and its products by the use of peptidoglycan-based molecules. The enzyme recognizes a minimum of four sugars in its substrate but can process a substrate comprised of a peptidoglycan of 20 sugars. The ultimate product of the reaction is N-acetylglucosamine-1,6-anhydro-N-acetylmuramic acid. The X-ray structure of this enzyme is reported for the first time. The enzyme is comprised of four domains, arranged within an annular conformation. The polymeric linear peptidoglycan substrate threads through the opening of the annulus, as it experiences turnover. Turnover of Bacterial Cell Wall by SltB3, a Multidomain Lytic Transglycosylase of Pseudomonas aeruginosa.,Lee M, Dominguez-Gil T, Hesek D, Mahasenan KV, Lastochkin E, Hermoso JA, Mobashery S ACS Chem Biol. 2016 Jun 17;11(6):1525-31. doi: 10.1021/acschembio.6b00194. Epub, 2016 Apr 11. PMID:27035839 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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