5ce5 | pdb_00005ce5

Probing the roles of two tryptophans surrounding the unique zinc coordination site in lipase family I.5

spinqualitylabelsall models 5ce5, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5ce5 is a 1 chain structure with sequence from Geobacillus thermocatenulatus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2Å Ligands: 1PE, CA, MPD, P15, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q59260_9BACI Publication Abstract from PubMed A unique zinc domain found in all of the identified members of the lipase family I.5 is surrounded by two conserved tryptophans (W61 and W212). In this study we investigated the role of these hydrophobic residues in thermostability and thermoactivity of the lipase from Bacillus thermocatenulatus (BTL2) taken as the representative of the family. Circular dichroism spectroscopy revealed that the secondary structure of BTL2 is conserved by the tryptophan mutations (W61A, W212A and W61A/W212A), and that W61 is located in a more rigid and less solvent exposed region than is W212. Thermal denaturation and optimal activity analyses pointed out that zinc induces thermostability and thermoactivity of BTL2, in which both tryptophans W61 and W212 play contributing roles. Molecular explanations describing the roles of these tryptophans were pursued by X-ray crystallography of the open form of the W61A mutant and molecular dynamics simulations which highlighted a critical function for W212 in zinc binding to the coordination site. This study reflects the potential use of hydrophobic amino acids in vicinity of metal coordination sites in lipase biocatalysts design. This article is protected by copyright. All rights reserved. Probing the roles of two tryptophans surrounding the unique zinc coordination site in lipase family I.5.,Timucin E, Cousido-Siah A, Mitschler A, Podjarny A, Sezerman OU Proteins. 2015 Nov 17. doi: 10.1002/prot.24961. PMID:26573720 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Lipase 3D Structures

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