1wco | pdb_00001wco

The solution structure of the nisin-lipid II complex

spinqualitylabelsall models 1wco Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 1wco is a 2 chain structure with sequence from Lactococcus lactis and Monarthropalpus flavus. This structure supersedes the now removed PDB entry 1uzt. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Solution NMR Ligands: DAL, DBB, DBU, DHA, FDF, FGA, MUB, NAG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function LANZ_LACLL Lanthionine-containing peptide antibiotic (lantibiotic) active on Gram-positive bacteria. The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores. Publication Abstract from PubMed The emerging antibiotics-resistance problem has underlined the urgent need for novel antimicrobial agents. Lantibiotics (lanthionine-containing antibiotics) are promising candidates to alleviate this problem. Nisin, a member of this family, has a unique pore-forming activity against bacteria. It binds to lipid II, the essential precursor of cell wall synthesis. As a result, the membrane permeabilization activity of nisin is increased by three orders of magnitude. Here we report the solution structure of the complex of nisin and lipid II. The structure shows a novel lipid II-binding motif in which the pyrophosphate moiety of lipid II is primarily coordinated by the N-terminal backbone amides of nisin via intermolecular hydrogen bonds. This cage structure provides a rationale for the conservation of the lanthionine rings among several lipid II-binding lantibiotics. The structure of the pyrophosphate cage offers a template for structure-based design of novel antibiotics. The nisin-lipid II complex reveals a pyrophosphate cage that provides a blueprint for novel antibiotics.,Hsu ST, Breukink E, Tischenko E, Lutters MA, de Kruijff B, Kaptein R, Bonvin AM, van Nuland NA Nat Struct Mol Biol. 2004 Oct;11(10):963-7. Epub 2004 Sep 12. PMID:15361862 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Nisin

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