6qta | pdb_00006qta

Crystal structure of Rea1-MIDAS/Rsa4-UBL complex from Chaetomium thermophilum

spinqualitylabelsall models 6qta, resolution 1.89Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6qta is a 2 chain structure with sequence from Chaetomium thermophilum var. thermophilum DSM 1495. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.89Å Ligands: GOL, MG, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function G0SHE6_CHATD Nuclear chaperone required for maturation and nuclear export of pre-60S ribosome subunits.[PIRNR:PIRNR010340] Publication Abstract from PubMed The Rea1 AAA(+)-ATPase dislodges assembly factors from pre-60S ribosomes upon ATP hydrolysis, thereby driving ribosome biogenesis. Here, we present crystal structures of Rea1-MIDAS, the conserved domain at the tip of the flexible Rea1 tail, alone and in complex with its substrate ligands, the UBL domains of Rsa4 or Ytm1. These complexes have structural similarity to integrin alpha-subunit domains when bound to extracellular matrix ligands, which for integrin biology is a key determinant for force-bearing cell-cell adhesion. However, the presence of additional motifs equips Rea1-MIDAS for its tasks in ribosome maturation. One loop insert cofunctions as an NLS and to activate the mechanochemical Rea1 cycle, whereas an additional beta-hairpin provides an anchor to hold the ligand UBL domains in place. Our data show the versatility of the MIDAS fold for mechanical force transmission in processes as varied as integrin-mediated cell adhesion and mechanochemical removal of assembly factors from pre-ribosomes. Crystal structures of Rea1-MIDAS bound to its ribosome assembly factor ligands resembling integrin-ligand-type complexes.,Ahmed YL, Thoms M, Mitterer V, Sinning I, Hurt E Nat Commun. 2019 Jul 11;10(1):3050. doi: 10.1038/s41467-019-10922-6. PMID:31296859 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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