6ybu | pdb_00006ybu

Crystal structure of a native BcsE (349-523) RQ complex with c-di-GMP and ATP bound

spinqualitylabelsall models 6ybu, resolution 2.49Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6ybu is a 12 chain structure with sequence from Escherichia coli and Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.49Å Ligands: ATP, C2E, MG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function BCSE_ECOLI Binds bis-(3'-5') cyclic diguanylic acid (c-di-GMP), the ability to bind c-di-GMP is important for its function (PubMed:24942809). Publication Abstract from PubMed Many free-living and pathogenic enterobacteria secrete biofilm-promoting cellulose using a multicomponent, envelope-embedded Bcs secretion system under the control of intracellular second messenger c-di-GMP. The molecular understanding of system assembly and cellulose secretion has been largely limited to the crystallographic studies of a distantly homologous BcsAB synthase tandem and a low-resolution reconstruction of an assembled macrocomplex that encompasses most of the inner membrane and cytosolic subunits and features an atypical layered architecture. Here, we present cryo-EM structures of the assembled Bcs macrocomplex, as well as multiple crystallographic snapshots of regulatory Bcs subcomplexes. The structural and functional data uncover the mechanism of asymmetric secretion system assembly and periplasmic crown polymerization and reveal unexpected subunit stoichiometry, multisite c-di-GMP recognition, and ATP-dependent regulation. Architecture and regulation of an enterobacterial cellulose secretion system.,Abidi W, Zouhir S, Caleechurn M, Roche S, Krasteva PV Sci Adv. 2021 Jan 27;7(5). pii: 7/5/eabd8049. doi: 10.1126/sciadv.abd8049. Print , 2021 Jan. PMID:33563593 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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