3ii2 | pdb_00003ii2

Structure of ORF157 from Acidianus Filamentous Virus 1

spinqualitylabelsall models 3ii2, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3ii2 is a 1 chain structure with sequence from Acidianus filamentous virus 1. This structure supersedes the now removed PDB entry 2oq8. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2Å Ligands: CL, GOL, HG, NI Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Y157_AFV1Y Publication Abstract from PubMed Acidianus filamentous virus 1 (AFV1) (Lipothrixviridae) is an enveloped filamentous virus that was characterized from a crenarchaeal host. It infects Acidianus species that thrive in the acidic hot springs (>85 degrees C and pH <3) of Yellowstone National Park, WY. The AFV1 20.8-kb, linear, double-stranded DNA genome encodes 40 putative open reading frames whose sequences generally show little similarity to other genes in the sequence databases. Because three-dimensional structures are more conserved than sequences and hence are more effective at revealing function, we set out to determine protein structures from putative AFV1 open reading frames (ORF). The crystal structure of ORF157 reveals an alpha+beta protein with a novel fold that remotely resembles the nucleotidyltransferase topology. In vitro, AFV1-157 displays a nuclease activity on linear double-stranded DNA. Alanine substitution mutations demonstrated that E86 is essential to catalysis. AFV1-157 represents a novel class of nuclease, but its exact role in vivo remains to be determined. ORF157 from the archaeal virus Acidianus filamentous virus 1 defines a new class of nuclease.,Goulet A, Pina M, Redder P, Prangishvili D, Vera L, Lichiere J, Leulliot N, van Tilbeurgh H, Ortiz-Lombardia M, Campanacci V, Cambillau C J Virol. 2010 May;84(10):5025-31. Epub 2010 Mar 3. PMID:20200253 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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