3j3x | pdb_00003j3x

Independent reconstruction of Mm-cpn cryo-EM density map from half dataset in the closed state (training map)

Loading... Status: waiting for Mol* initialization Structural highlights 3j3x is a 16 chain structure with sequence from Methanococcus maripaludis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 4.3Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q877G8_METMI Publication Abstract from PubMed An increasing number of cryo-electron microscopy (cryo-EM) density maps are being generated with suitable resolution to trace the protein backbone and guide sidechain placement. Generating and evaluating atomic models based on such maps would be greatly facilitated by independent validation metrics for assessing the fit of the models to the data. We describe such a metric based on the fit of atomic models with independent test maps from single particle reconstructions not used in model refinement. The metric provides a means to determine the proper balance between the fit to the density and model energy and stereochemistry during refinement, and is likely to be useful in determining values of model building and refinement metaparameters quite generally. Cryo-EM model validation using independent map reconstructions.,Dimaio F, Zhang J, Chiu W, Baker D Protein Sci. 2013 Jun;22(6):865-8. doi: 10.1002/pro.2267. PMID:23592445 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Chaperonin 3D structures

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