3j5y | pdb_00003j5y

Structure of the mammalian ribosomal pre-termination complex associated with eRF1-eRF3-GDPNP

Loading... Status: waiting for Mol* initialization Structural highlights 3j5y is a 4 chain structure with sequence from Homo sapiens and Oryctolagus cuniculus. This structure supersedes the now removed PDB entry 3j2k. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 9.7Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function ERF1_HUMAN Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Publication Abstract from PubMed Eukaryotic translation termination results from the complex functional interplay between two release factors, eRF1 and eRF3, in which GTP hydrolysis by eRF3 couples codon recognition with peptidyl-tRNA hydrolysis by eRF1. Here, we present a cryo-electron microscopy structure of pre-termination complexes associated with eRF1*eRF3*GDPNP at 9.7 -A resolution, which corresponds to the initial pre-GTP hydrolysis stage of factor attachment and stop codon recognition. It reveals the ribosomal positions of eRFs and provides insights into the mechanisms of stop codon recognition and triggering of eRF3's GTPase activity. Structure of the mammalian ribosomal pre-termination complex associated with eRF1*eRF3*GDPNP.,des Georges A, Hashem Y, Unbehaun A, Grassucci RA, Taylor D, Hellen CU, Pestova TV, Frank J Nucleic Acids Res. 2013 Dec 11. PMID:24335085 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Transfer RNA (tRNA)

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