3j6x | pdb_00003j6x

S. cerevisiae 80S ribosome bound with Taura syndrome virus (TSV) IRES, 5 degree rotation (Class II)

Loading... Status: waiting for Mol* initialization Structural highlights 3j6x is a 10 chain structure with sequence from Saccharomyces cerevisiae W303. This structure supersedes the now removed PDB entries 1vw7, 1vw8 and 1vw9. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 6.1Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RL13A_YEAST Publication Abstract from PubMed In cap-dependent translation initiation, the open reading frame (ORF) of mRNA is established by the placement of the AUG start codon and initiator tRNA in the ribosomal peptidyl (P) site. Internal ribosome entry sites (IRESs) promote translation of mRNAs in a cap-independent manner. We report two structures of the ribosome-bound Taura syndrome virus (TSV) IRES belonging to the family of Dicistroviridae intergenic IRESs. Intersubunit rotational states differ in these structures, suggesting that ribosome dynamics play a role in IRES translocation. Pseudoknot I of the IRES occupies the ribosomal decoding center at the aminoacyl (A) site in a manner resembling that of the tRNA anticodon-mRNA codon. The structures reveal that the TSV IRES initiates translation by a previously unseen mechanism, which is conceptually distinct from initiator tRNA-dependent mechanisms. Specifically, the ORF of the IRES-driven mRNA is established by the placement of the preceding tRNA-mRNA-like structure in the A site, whereas the 40S P site remains unoccupied during this initial step. Taura syndrome virus IRES initiates translation by binding its tRNA-mRNA-like structural element in the ribosomal decoding center.,Koh CS, Brilot AF, Grigorieff N, Korostelev AA Proc Natl Acad Sci U S A. 2014 Jun 24;111(25):9139-44. doi:, 10.1073/pnas.1406335111. Epub 2014 Jun 9. PMID:24927574 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ribosome 3D structures 3D sructureseceptor for activated protein kinase C 1

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