7aar | pdb_00007aar
sugar/H+ symporter STP10 in inward open conformation
Structural highlights
FunctionSTP10_ARATH Mediates an active uptake of hexoses, probably by sugar/hydrogen symport. Publication Abstract from PubMedSugars are essential sources of energy and carbon and also function as key signalling molecules in plants. Sugar transport proteins (STP) are proton-coupled symporters responsible for uptake of glucose from the apoplast into plant cells. They are integral to organ development in symplastically isolated tissues such as seed, pollen and fruit. Additionally, STPs play a vital role in plant responses to stressors such as dehydration and prevalent fungal infections like rust and mildew. Here we present a structure of Arabidopsis thaliana STP10 in the inward-open conformation at 2.6 A resolution and a structure of the outward-occluded conformation at improved 1.8 A resolution, both with glucose and protons bound. The two structures describe key states in the STP transport cycle. Together with molecular dynamics simulations that establish protonation states and biochemical analysis, they pinpoint structural elements, conserved in all STPs, that clarify the basis of proton-to-glucose coupling. These results advance our understanding of monosaccharide uptake, which is essential for plant organ development, and set the stage for bioengineering strategies in crops. Molecular mechanism of sugar transport in plants unveiled by structures of glucose/H(+) symporter STP10.,Bavnhoj L, Paulsen PA, Flores-Canales JC, Schiott B, Pedersen BP Nat Plants. 2021 Sep 23. pii: 10.1038/s41477-021-00992-0. doi:, 10.1038/s41477-021-00992-0. PMID:34556835[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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