7o54 | pdb_00007o54

Crystal structure of the carbonic anhydrase-like domain of CcmM in complex with the C-terminal 17 residues of CcaA from Synechococcus elongatus (strain PCC 7942)

spinqualitylabelsall models 7o54, resolution 1.63Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7o54 is a 2 chain structure with sequence from Synechococcus elongatus PCC 7942 = FACHB-805. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.63Å Ligands: CL, NI Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function CCMM_SYNE7 The presence of two potential DNA-binding regions suggests this protein may be a transcriptional regulator. Publication Abstract from PubMed Carboxysomes in cyanobacteria enclose the enzymes Rubisco and carbonic anhydrase to optimize photosynthetic carbon fixation. Understanding carboxysome assembly has implications in agricultural biotechnology. Here we analyzed the role of the scaffolding protein CcmM of the beta-cyanobacterium Synechococcus elongatus PCC 7942 in sequestrating the hexadecameric Rubisco and the tetrameric carbonic anhydrase, CcaA. We find that the trimeric CcmM, consisting of gammaCAL oligomerization domains and linked small subunit-like (SSUL) modules, plays a central role in mediation of pre-carboxysome condensate formation through multivalent, cooperative interactions. The gammaCAL domains interact with the C-terminal tails of the CcaA subunits and additionally mediate a head-to-head association of CcmM trimers. Interestingly, SSUL modules, besides their known function in recruiting Rubisco, also participate in intermolecular interactions with the gammaCAL domains, providing further valency for network formation. Our findings reveal the mechanism by which CcmM functions as a central organizer of the pre-carboxysome multiprotein matrix, concentrating the core components Rubisco and CcaA before beta-carboxysome shell formation. Scaffolding protein CcmM directs multiprotein phase separation in beta-carboxysome biogenesis.,Zang K, Wang H, Hartl FU, Hayer-Hartl M Nat Struct Mol Biol. 2021 Nov;28(11):909-922. doi: 10.1038/s41594-021-00676-5., Epub 2021 Nov 10. PMID:34759380 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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