7p9e | pdb_00007p9e

Chlamydomonas reinhardtii NADPH Dependent Thioredoxin Reductase 1 domain CS mutant

spinqualitylabelsall models 7p9e, resolution 2.36Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7p9e is a 2 chain structure with sequence from Chlamydomonas reinhardtii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.36Å Ligands: FAD, P4G Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A2K3E888_CHLRE Publication Abstract from PubMed In plant chloroplasts, thiol regulation is driven by two systems. One relies on the activity of thioredoxins through their light dependent reduction by ferredoxin via a ferredoxin-thioredoxin reductase (FTR). In the other system, a NADPH-dependent redox regulation is driven by a NADPH-thioredoxin reductase C (NTRC). While the thioredoxin system has been deeply studied, a more thorough understanding of the function of this plant specific NTRC is desirable. NTRC is a single polypeptide harbouring a thioredoxin domain (Trx) at the C-terminus of a NADPH-dependent Thioredoxin reductase (TrxR). To provide functional and structural insights, we studied the crystal structure of the TrxR domain of the NTRC from Chlamydomonas reinhardtii (CrNTRC, Cre01.g054150.t1.2) and its Cys136Ser (C136S) mutant, which is characterized by the mutation of the resolving cysteine in the active site of the TrxR domain. Furthermore, we confirmed the role of NTRC as electron donor for 2-Cys peroxiredoxin (PRX) also in C. reinhardtii. The structural data of TrxR were employed to develop a scheme of action which addresses electron transfer between TrxR and Trx of NTRC and between NTRC and its substrates. Structural analysis revealed a novel conformation of the NTRC reductase domain from Chlamydomonas reinhardtii.,Marchetti GM, Fusser F, Singh RK, Brummel M, Koch O, Kummel D, Hippler M J Struct Biol. 2021 Dec 30;214(1):107829. doi: 10.1016/j.jsb.2021.107829. PMID:34974142 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Thioredoxin reductase 3D structures

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