7pgb | pdb_00007pgb

NaV_Ae1/Sp1CTD_pore-SAT09 complex

spinqualitylabelsall models 7pgb, resolution 3.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7pgb is a 30 chain structure with sequence from Alkalilimnicola ehrlichii MLHE-1, Homo sapiens and Ruegeria pomeroyi DSS-3. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.6Å Ligands: 1PE, 4NB, BHC, D12, GOL, LHG, LNK, OCT, PE4, R16 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q0ABW0_ALKEH F7IVA8_RUEPO Publication Abstract from PubMed Every voltage-gated ion channel (VGIC) has a pore domain (PD) made from four subunits, each comprising an antiparallel transmembrane helix pair bridged by a loop. The extent to which PD subunit structure requires quaternary interactions is unclear. Here, we present crystal structures of a set of bacterial voltage-gated sodium channel (BacNaV) 'pore only' proteins that reveal a surprising collection of non-canonical quaternary arrangements in which the PD tertiary structure is maintained. This context-independent structural robustness, supported by molecular dynamics simulations, indicates that VGIC-PD tertiary structure is independent of quaternary interactions. This fold occurs throughout the VGIC superfamily and in diverse transmembrane and soluble proteins. Strikingly, characterization of PD subunit-binding Fabs indicates that non-canonical quaternary PD conformations can occur in full-length VGICs. Together, our data demonstrate that the VGIC-PD is an autonomously folded unit. This property has implications for VGIC biogenesis, understanding functional states, de novo channel design, and VGIC structural origins. Quaternary structure independent folding of voltage-gated ion channel pore domain subunits.,Arrigoni C, Lolicato M, Shaya D, Rohaim A, Findeisen F, Fong LK, Colleran CM, Dominik P, Kim SS, Schuermann JP, DeGrado WF, Grabe M, Kossiakoff AA, Minor DL Jr Nat Struct Mol Biol. 2022 Jun 2. pii: 10.1038/s41594-022-00775-x. doi:, 10.1038/s41594-022-00775-x. PMID:35655098 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Antibody 3D structures Ion channels 3D structures

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