7ppv | pdb_00007ppv

Structure of diFe-Sulerythrin at 2.70 MGy total absorbed dose

spinqualitylabelsall models 7ppv, resolution 1.36Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7ppv is a 2 chain structure with sequence from Sulfurisphaera tokodaii str. 7. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.36Å Ligands: CL, FE, OH Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function F9VPE5_SULTO Publication Abstract from PubMed Protein-mediated redox reactions play a critical role in many biological processes and often occur at centres that contain metal ions as cofactors. In order to understand the exact mechanisms behind these reactions it is important to not only characterize the three-dimensional structures of these proteins and their cofactors, but also to identify the oxidation states of the cofactors involved and to correlate this knowledge with structural information. The only suitable approach for this based on crystallographic measurements is spatially resolved anomalous dispersion (SpReAD) refinement, a method that has been used previously to determine the redox states of metals in iron-sulfur cluster-containing proteins. In this article, the feasibility of this approach for small, non-iron-sulfur redox centres is demonstrated by employing SpReAD analysis to characterize Sulfolobus tokodaii sulerythrin, a ruberythrin-like protein that contains a binuclear metal centre. Differences in oxidation states between the individual iron ions of the binuclear metal centre are revealed in sulerythrin crystals treated with H2O2. Furthermore, data collection at high X-ray doses leads to photoreduction of this metal centre, showing that careful control of the total absorbed dose is a prerequisite for successfully determining the oxidation state through SpReAD analysis. Determining the oxidation state of elements by X-ray crystallography.,Lennartz F, Jeoung JH, Ruenger S, Dobbek H, Weiss MS Acta Crystallogr D Struct Biol. 2022 Feb 1;78(Pt 2):238-247. doi:, 10.1107/S2059798321013048. Epub 2022 Jan 24. PMID:35102889 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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