Structural highlights
Function
OTEMO_PSEPU Involved in the degradation of (+)-camphor. Catalyzes the lactonization of 2-oxo-delta(3)-4,5, 5-trimethylcyclopentenylacetyl-CoA (OT-CoA), a key intermediate in the metabolism of camphor. 2-Oxocyclopentyl ethyl acetate is also a good substrate, as is 2-oxocyclohexyl ethyl acetate and methyl-substituted cyclohexanones, but free acid is a poor substrate.[1] [2] [3]
References
- ↑ Leisch H, Shi R, Grosse S, Morley K, Bergeron H, Cygler M, Iwaki H, Hasegawa Y, Lau PC. Camphor Pathway 2-Oxo-{Delta}3-4,5,5-trimethylcyclopentenylacetyl-CoA Monooxygenase of Pseudomonas putida ATCC 17453: Cloning, Baeyer-Villiger Biooxidations, and Structures. Appl Environ Microbiol. 2012 Jan 20. PMID:22267661 doi:10.1128/AEM.07694-11
- ↑ Kadow M, Loschinski K, Sass S, Schmidt M, Bornscheuer UT. Completing the series of BVMOs involved in camphor metabolism of Pseudomonas putida NCIMB 10007 by identification of the two missing genes, their functional expression in E. coli, and biochemical characterization. Appl Microbiol Biotechnol. 2012 Oct;96(2):419-29. PMID:22286514 doi:https://dx.doi.org/10.1007/s00253-011-3859-1
- ↑ Ougham HJ, Taylor DG, Trudgill PW. Camphor revisited: involvement of a unique monooxygenase in metabolism of 2-oxo-delta 3-4,5,5-trimethylcyclopentenylacetic acid by Pseudomonas putida. J Bacteriol. 1983 Jan;153(1):140-52. PMID:6848481 doi:10.1128/jb.153.1.140-152.1983
