2vyf | pdb_00002vyf

Crystal Structure of the DnaC

spinqualitylabelsall models 2vyf, resolution 3.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2vyf is a 2 chain structure with sequence from Geobacillus kaustophilus HTA426. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.6Å Ligands: AU Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q5KU75_GEOKA Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed DNA helicases are motor proteins that play essential roles in DNA replication, repair and recombination. In the replicative hexameric helicase, the fundamental reaction is the unwinding of duplex DNA; however, our understanding of this function remains vague due to insufficient structural information. Here, we report two crystal structures of the DnaB-family replicative helicase from Geobacillus kaustophilus HTA426 (GkDnaC) in the apo-form and bound to single-stranded DNA (ssDNA). The GkDnaC-ssDNA complex structure reveals that three symmetrical basic grooves on the interior surface of the hexamer individually encircle ssDNA. The ssDNA-binding pockets in this structure are directed toward the N-terminal domain collar of the hexameric ring, thus orienting the ssDNA toward the DnaG primase to facilitate the synthesis of short RNA primers. These findings provide insight into the mechanism of ssDNA binding and provide a working model to establish a novel mechanism for DNA translocation at the replication fork. The crystal structure of a replicative hexameric helicase DnaC and its complex with single-stranded DNA.,Lo YH, Tsai KL, Sun YJ, Chen WT, Huang CY, Hsiao CD Nucleic Acids Res. 2008 Dec 15. PMID:19074952 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also DnaC helicase loader Helicase 3D structures

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