4v5x | pdb_00004v5x

The cryo-EM structure of a 3D DNA-origami object

Loading... Status: waiting for Mol* initialization Structural highlights 4v5x is a 10 chain structure with sequence from Escherichia virus M13 and Synthetic construct. This structure supersedes the now removed PDB entries 2ymf, 2ymg, 2ymh, 2ymi and 2ymr. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 11.5Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed A key goal for nanotechnology is to design synthetic objects that may ultimately achieve functionalities known today only from natural macromolecular complexes. Molecular self-assembly with DNA has shown potential for creating user-defined 3D scaffolds, but the level of attainable positional accuracy has been unclear. Here we report the cryo-EM structure and a full pseudoatomic model of a discrete DNA object that is almost twice the size of a prokaryotic ribosome. The structure provides a variety of stable, previously undescribed DNA topologies for future use in nanotechnology and experimental evidence that discrete 3D DNA scaffolds allow the positioning of user-defined structural motifs with an accuracy that is similar to that observed in natural macromolecules. Thereby, our results indicate an attractive route to fabricate nanoscale devices that achieve complex functionalities by DNA-templated design steered by structural feedback. Cryo-EM structure of a 3D DNA-origami object.,Bai XC, Martin TG, Scheres SH, Dietz H Proc Natl Acad Sci U S A. 2012 Nov 19. PMID:23169645 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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