4v8l | pdb_00004v8l

Cryo-EM Structure of the Mycobacterial Fatty Acid Synthase

Loading... Status: waiting for Mol* initialization Structural highlights 4v8l is a 6 chain structure with sequence from Mycolicibacterium smegmatis. This structure supersedes the now removed PDB entries 3zen and 4b3y. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 7.5Å Ligands: FMN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0R1H7_MYCS2 Publication Abstract from PubMed The mycobacterial fatty acid synthase (FAS) complex is a giant 2.0-MDa alpha(6) homohexameric multifunctional enzyme that catalyzes synthesis of fatty acid precursors of mycolic acids, which are major components of the cell wall in Mycobacteria and play an important role in pathogenicity. Here, we present a three-dimensional reconstruction of the Mycobacterium smegmatis FAS complex at 7.5A, highly homologous to the Mycobacterium tuberculosis multienzyme, by cryo-electron microscopy. Based on the obtained structural data, which allowed us to identify secondary-structure elements, and sequence homology with the fungal FAS, we generated an accurate architectural model of the complex. The FAS system from Mycobacteria resembles a minimized version of the fungal FAS with much larger openings in the reaction chambers. These architectural features of the mycobacterial FAS may be important for the interaction with mycolic acid processing and condensing enzymes that further modify the precursors produced by FAS and for autoactivation of the FAS complex. 7.5-A Cryo-EM Structure of the Mycobacterial Fatty Acid Synthase.,Boehringer D, Ban N, Leibundgut M J Mol Biol. 2013 Jan 3. pii: S0022-2836(12)00969-2. doi:, 10.1016/j.jmb.2012.12.021. PMID:23291528 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Fatty acid synthase 3D structures

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