6flk | pdb_00006flk
Crystal structure of Cep120 C2C domain
Structural highlights
FunctionCE120_HUMAN Plays a role in the microtubule-dependent coupling of the nucleus and the centrosome. Involved in the processes that regulate centrosome-mediated interkinetic nuclear migration (INM) of neural progenitors and for proper positioning of neurons during brain development. Also implicated in the migration and selfrenewal of neural progenitors. May play a role in centriole duplication during mitosis (By similarity). Publication Abstract from PubMedCentrioles are microtubule-based structures that play essential roles in cell division and cilia biogenesis. Cep120 is an important protein for correct centriole formation and mutations in the Cep120 gene cause severe human diseases like Joubert syndrome and complex ciliopathies. Here, we show that Cep120 contains three consecutive C2 domains that are followed by a coiled-coil dimerization domain. Surprisingly, unlike the classical C2 domains, all three Cep120 C2 domains lack calcium- and phospholipid-binding activities. However, biophysical and biochemical assays revealed that the N-terminal Cep120 C2 domain (C2A) binds to both tubulin and microtubules, and promotes microtubule formation. Structural analyses coupled with mutagenesis identified a highly conserved, positively charged residue patch on the surface of Cep120 C2A, which mediates the interaction with tubulin and microtubules. Together, our results establish Cep120 C2A as a unique microtubule-binding domain. They further provide insights into the molecular mechanism of Cep120 during centriole biogenesis. Cep120 promotes microtubule formation through a unique tubulin binding C2 domain.,Sharma A, Gerard SF, Olieric N, Steinmetz MO J Struct Biol. 2018 Feb 1. pii: S1047-8477(18)30024-8. doi:, 10.1016/j.jsb.2018.01.009. PMID:29398280[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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