8wks | pdb_00008wks

Cryo-EM structure of DSR2-TUBE complex

spinqualitylabelsall models 8wks, resolution 3.58Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8wks is a 8 chain structure with sequence from Bacillus subtilis subsp. natto BEST195 and Siphoviridae sp. ct0106. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.58Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function D4G637_BACNB Publication Abstract from PubMed Defense-associated sirtuin 2 (DSR2) systems are widely distributed across prokaryotic genomes, providing robust protection against phage infection. DSR2 recognizes phage tail tube proteins and induces abortive infection by depleting intracellular NAD(+), a process that is counteracted by another phage-encoded protein, DSR Anti Defense 1 (DSAD1). Here, we present cryo-EM structures of Bacillus subtilis DSR2 in its apo, Tube-bound, and DSAD1-bound states. DSR2 assembles into an elongated tetramer, with four NADase catalytic modules clustered in the center and the regulatory-sensing modules distributed at four distal corners. Interestingly, monomeric Tube protein, rather than its oligomeric states, docks at each corner of the DSR2 tetramer to form a 4:4 DSR2-Tube assembly, which is essential for DSR2 NADase activity. DSAD1 competes with Tube for binding to DSR2 by occupying an overlapping region, thereby inhibiting DSR2 immunity. Thus, our results provide important insights into the assembly, activation and inhibition of the DSR2 anti-phage defense system. Molecular basis of bacterial DSR2 anti-phage defense and viral immune evasion.,Huang J, Zhu K, Gao Y, Ye F, Li Z, Ge Y, Liu S, Yang J, Gao A Nat Commun. 2024 May 10;15(1):3954. doi: 10.1038/s41467-024-48291-4. PMID:38729958 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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