6s3k | pdb_00006s3k

KimA from Bacillus subtilis in inward-facing, occluded state

Loading... Status: waiting for Mol* initialization Structural highlights 6s3k is a 2 chain structure with sequence from Bacillus subtilis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.7Å Ligands: K Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function KIMA_BACSU High-affinity potassium transporter (PubMed:28420751, PubMed:32005818). Functions as a K(+)/H(+) symporter (PubMed:32005818). Publication Abstract from PubMed Potassium homeostasis is vital for all organisms, but is challenging in single-celled organisms like bacteria and yeast and immobile organisms like plants that constantly need to adapt to changing external conditions. KUP transporters facilitate potassium uptake by the co-transport of protons. Here, we uncover the molecular basis for transport in this widely distributed family. We identify the potassium importer KimA from Bacillus subtilis as a member of the KUP family, demonstrate that it functions as a K(+)/H(+) symporter and report a 3.7 A cryo-EM structure of the KimA homodimer in an inward-occluded, trans-inhibited conformation. By introducing point mutations, we identify key residues for potassium and proton binding, which are conserved among other KUP proteins. Structural basis of proton-coupled potassium transport in the KUP family.,Tascon I, Sousa JS, Corey RA, Mills DJ, Griwatz D, Aumuller N, Mikusevic V, Stansfeld PJ, Vonck J, Hanelt I Nat Commun. 2020 Jan 31;11(1):626. doi: 10.1038/s41467-020-14441-7. PMID:32005818 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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