2dda | pdb_00002dda
Crystal structure of pseudechetoxin from Pseudechis australis
Structural highlights
FunctionCRVP_PSEAU Blocks olfactory (CNGA2) and retinal (CNGA1) cyclic nucleotide-gated (CNG) ion channel currents. Does not inhibit retinal (CNGA3) currents. It forms high-affinity contacts with the pore turret region and most likely inhibits CNG channel current by blocking the external entrance to the transmembrane pore. Is really more potent that Pseudecin. Does not affect neither depolarization- nor caffeine-induced contraction arterial smooth muscle.[1] [2] [3] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedCyclic nucleotide-gated (CNG) ion channels play pivotal roles in sensory transduction by retinal photoreceptors and olfactory neurons. The elapid snake toxins pseudechetoxin (PsTx) and pseudecin (Pdc) are the only known protein blockers of CNG channels. These toxins belong to a cysteine-rich secretory protein (CRISP) family containing an N-terminal pathogenesis-related proteins of group 1 (PR-1) domain and a C-terminal cysteine-rich domain (CRD). PsTx and Pdc are highly homologous proteins, but their blocking affinities on CNG channels are different: PsTx blocks both the olfactory and retinal channels with approximately 15-30-fold higher affinity than Pdc. To gain further insights into their structure and function, the crystal structures of PsTx, Pdc and Zn2+-bound Pdc were determined. The structures revealed that most of the amino-acid-residue differences between PsTx and Pdc are located around the concave surface formed between the PR-1 domain and the CRD, suggesting that the concave surface is functionally important for CNG-channel binding and inhibition. A structural comparison in the presence and absence of Zn2+ ion demonstrated that the concave surface can open and close owing to movement of the CRD upon Zn2+ binding. The data suggest that PsTx and Pdc occlude the pore entrance and that the dynamic motion of the concave surface facilitates interaction with the CNG channels. Structures of pseudechetoxin and pseudecin, two snake-venom cysteine-rich secretory proteins that target cyclic nucleotide-gated ion channels: implications for movement of the C-terminal cysteine-rich domain.,Suzuki N, Yamazaki Y, Brown RL, Fujimoto Z, Morita T, Mizuno H Acta Crystallogr D Biol Crystallogr. 2008 Oct;64(Pt 10):1034-42. Epub 2008, Sep 19. PMID:18931410[4] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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