9b33 | pdb_00009b33

Structure of concanavalin A (ConA) dimer from the open-state structure of kainate receptor GluK2 in complex with agonist glutamate and positive allosteric modulator BPAM344 bound to one ConA dimer. Type II interface between GluK2 ligand-binding domain and ConA

spinqualitylabelsall models 9b33, resolution 4.07Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9b33 is a 2 chain structure with sequence from Canavalia ensiformis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 4.07Å Ligands: CA, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function CONV_CANCT D-mannose/D-glucose-binding lectin which binds alpha-methyl-D-mannoside, D-mannose and D-glucose in that order (PubMed:24519628, PubMed:27737777). Also binds to serum fetuin and ovalbumin (PubMed:24519628). Has hemagglutinating activity towards rabbit erythrocytes (PubMed:24519628). Is not toxic towards larvae of the brine shrimp Artemia (PubMed:24519628). Induces relaxation in rat endothelized aorta (PubMed:24519628). Shows a transient edematogenic effect in rat (PubMed:27737777). Publication Abstract from PubMed Kainate receptors, a subclass of ionotropic glutamate receptors, are tetrameric ligand-gated ion channels that mediate excitatory neurotransmission(1-4). Kainate receptors modulate neuronal circuits and synaptic plasticity during the development and function of the central nervous system and are implicated in various neurological and psychiatric diseases, including epilepsy, depression, schizophrenia, anxiety and autism(5-11). Although structures of kainate receptor domains and subunit assemblies are available(12-18), the mechanism of kainate receptor gating remains poorly understood. Here we present cryo-electron microscopy structures of the kainate receptor GluK2 in the presence of the agonist glutamate and the positive allosteric modulators lectin concanavalin A and BPAM344. Concanavalin A and BPAM344 inhibit kainate receptor desensitization and prolong activation by acting as a spacer between the amino-terminal and ligand-binding domains and a stabilizer of the ligand-binding domain dimer interface, respectively. Channel opening involves the kinking of all four pore-forming M3 helices. Our structures reveal the molecular basis of kainate receptor gating, which could guide the development of drugs for treatment of neurological disorders. Kainate receptor channel opening and gating mechanism.,Gangwar SP, Yelshanskaya MV, Nadezhdin KD, Yen LY, Newton TP, Aktolun M, Kurnikova MG, Sobolevsky AI Nature. 2024 May 22. doi: 10.1038/s41586-024-07475-0. PMID:38778115 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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