8fty | pdb_00008fty

Crystal structure of the carotenoid isomerooxygenase, NinaB

spinqualitylabelsall models 8fty, resolution 1.95Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8fty is a 8 chain structure with sequence from Trichoplusia ni. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.95Å Ligands: CAC, CL, CSD, CSO, FE2, MPD, NA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The retinal light response in animals originates from the photoisomerization of an opsin-coupled 11-cis-retinaldehyde chromophore. This visual chromophore is enzymatically produced through the action of carotenoid cleavage dioxygenases. Vertebrates require two carotenoid cleavage dioxygenases, beta-carotene oxygenase 1 and retinal pigment epithelium 65 (RPE65), to form 11-cis-retinaldehyde from carotenoid substrates, whereas invertebrates such as insects use a single enzyme known as Neither Inactivation Nor Afterpotential B (NinaB). RPE65 and NinaB couple trans-cis isomerization with hydrolysis and oxygenation, respectively, but the mechanistic relationship of their isomerase activities remains unknown. Here we report the structure of NinaB, revealing details of its active site architecture and mode of membrane binding. Structure-guided mutagenesis studies identify a residue cluster deep within the NinaB substrate-binding cleft that controls its isomerization activity. Our data demonstrate that isomerization activity is mediated by distinct active site regions in NinaB and RPE65-an evolutionary convergence that deepens our understanding of visual system diversity. Carotenoid cleavage enzymes evolved convergently to generate the visual chromophore.,Solano YJ, Everett MP, Dang KS, Abueg J, Kiser PD Nat Chem Biol. 2024 Jun;20(6):779-788. doi: 10.1038/s41589-024-01554-z. Epub 2024 , Feb 14. PMID:38355721 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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