7nnu | pdb_00007nnu

Cryo-EM structure of the folate-specific ECF transporter complex in MSP2N2 lipid nanodiscs

spinqualitylabelsall models 7nnu, resolution 2.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7nnu is a 4 chain structure with sequence from Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 = JCM 1002. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 2.7Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q1G929_LACDA Publication Abstract from PubMed Energy-coupling factor (ECF)-type transporters are small, asymmetric membrane protein complexes ( approximately 115 kDa) that consist of a membrane-embedded, substrate-binding protein (S component) and a tripartite ATP-hydrolyzing module (ECF module). They import micronutrients into bacterial cells and have been proposed to use a highly unusual transport mechanism, in which the substrate is dragged across the membrane by a toppling motion of the S component. However, it remains unclear how the lipid bilayer could accommodate such a movement. Here, we used cryogenic electron microscopy at 200 kV to determine structures of a folate-specific ECF transporter in lipid nanodiscs and detergent micelles at 2.7- and 3.4-A resolution, respectively. The structures reveal an irregularly shaped bilayer environment around the membrane-embedded complex and suggest that toppling of the S component is facilitated by protein-induced membrane deformations. In this way, structural remodeling of the lipid bilayer environment is exploited to guide the transport process. Insights into the bilayer-mediated toppling mechanism of a folate-specific ECF transporter by cryo-EM.,Thangaratnarajah C, Rheinberger J, Paulino C, Slotboom DJ Proc Natl Acad Sci U S A. 2021 Aug 24;118(34):e2105014118. doi: , 10.1073/pnas.2105014118. PMID:34408021 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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