7zlv | pdb_00007zlv
Tail tip of siphophage T5 : central fibre protein pb4
Structural highlights
FunctionFIBC_BPT5 Together with BHP-pb3, forms the central straight fiber, bearing the receptor binding protein RBP-pb5 at its C-terminus (PubMed:36961893). Upon RBP-pb5 binding to the host receptor FhuA, the central fiber reorganizes and undergoes structural rearrangements that lead to its bending, bringing BHP-pb3 close to the host membrane for anchoring (PubMed:36961893).[1] Publication Abstract from PubMedMost bacteriophages present a tail allowing host recognition, cell wall perforation, and viral DNA channeling from the capsid to the infected bacterium cytoplasm. The majority of tailed phages bear a long flexible tail (Siphoviridae) at the tip of which receptor binding proteins (RBPs) specifically interact with their host, triggering infection. In siphophage T5, the unique RBP is located at the extremity of a central fiber. We present the structures of T5 tail tip, determined by cryo-electron microscopy before and after interaction with its E. coli receptor, FhuA, reconstituted into nanodisc. These structures bring out the important conformational changes undergone by T5 tail tip upon infection, which include bending of T5 central fiber on the side of the tail tip, tail anchoring to the membrane, tail tube opening, and formation of a transmembrane channel. The data allow to detail the first steps of an otherwise undescribed infection mechanism. Structural basis of bacteriophage T5 infection trigger and E. coli cell wall perforation.,Linares R, Arnaud CA, Effantin G, Darnault C, Epalle NH, Boeri Erba E, Schoehn G, Breyton C Sci Adv. 2023 Mar 24;9(12):eade9674. doi: 10.1126/sciadv.ade9674. Epub 2023 Mar , 24. PMID:36961893[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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