8arh | pdb_00008arh
In situ subtomogram average of Vaccinia virus (WR) D13 lattice, on immature virions
Structural highlights
FunctionPG125_VACCW Scaffold protein which forms a transitory spherical honeycomb lattice providing curvature and rigidity to the convex membrane of crescent and immature virions (IV). This association occurs concomitantly with viral membrane formation. Targeted by the drug rifampicin, which prevents the formation of this lattice, and hence virus morphogenesis. In the presence of rifampicin, irregularly shaped membranes that lack the honeycomb layer accumulate around areas of electron-dense viroplasm. This layer is lost from virions during maturation from IV to mature virion (MV), through the proteolysis of OPG158 N-terminus.[1] [2] Publication Abstract from PubMedDuring its cytoplasmic replication, vaccinia virus assembles non-infectious spherical immature virions (IV) coated by a viral D13 lattice. Subsequently, IV mature into infectious brick-shaped intracellular mature virions (IMV) that lack D13. Here, we performed cryo-electron tomography (cryo-ET) of frozen-hydrated vaccinia-infected cells to structurally characterise the maturation process in situ. During IMV formation, a new viral core forms inside IV with a wall consisting of trimeric pillars arranged in a new pseudohexagonal lattice. This lattice appears as a palisade in cross-section. As maturation occurs, which involves a 50% reduction in particle volume, the viral membrane becomes corrugated as it adapts to the newly formed viral core in a process that does not appear to require membrane removal. Our study suggests that the length of this core is determined by the D13 lattice and that the consecutive D13 and palisade lattices control virion shape and dimensions during vaccinia assembly and maturation. A succession of two viral lattices drives vaccinia virus assembly.,Hernandez-Gonzalez M, Calcraft T, Nans A, Rosenthal PB, Way M PLoS Biol. 2023 Mar 2;21(3):e3002005. doi: 10.1371/journal.pbio.3002005. , eCollection 2023 Mar. PMID:36862727[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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