8clk | pdb_00008clk

TFIIIC TauA complex

spinqualitylabelsall models 8clk, resolution 3.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8clk is a 4 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.5Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function TF3C6_HUMAN Involved in RNA polymerase III-mediated transcription. Integral, tightly associated component of the DNA-binding TFIIIC2 subcomplex that directly binds tRNA and virus-associated RNA promoters. Publication Abstract from PubMed Transcription factor (TF) IIIC recruits RNA polymerase (Pol) III to most of its target genes. Recognition of intragenic A- and B-box motifs in transfer RNA (tRNA) genes by TFIIIC modules tauA and tauB is the first critical step for tRNA synthesis but is mechanistically poorly understood. Here, we report cryo-electron microscopy structures of the six-subunit human TFIIIC complex unbound and bound to a tRNA gene. The tauB module recognizes the B-box via DNA shape and sequence readout through the assembly of multiple winged-helix domains. TFIIIC220 forms an integral part of both tauA and tauB connecting the two subcomplexes via a ~550-amino acid residue flexible linker. Our data provide a structural mechanism by which high-affinity B-box recognition anchors TFIIIC to promoter DNA and permits scanning for low-affinity A-boxes and TFIIIB for Pol III activation. Structural insights into human TFIIIC promoter recognition.,Seifert-Davila W, Girbig M, Hauptmann L, Hoffmann T, Eustermann S, Muller CW Sci Adv. 2023 Jul 7;9(27):eadh2019. doi: 10.1126/sciadv.adh2019. Epub 2023 Jul 7. PMID:37418517 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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